Gfp Lc3 Cell Line

The lc3b protein is a good marker to assess the autophagic flux since.

Gfp lc3 cell line.

However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs. Production details egfp lc3 rat plasmid was transfected into hek293a cells. This cell line uses gfp lc3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods. Single cell clones were selected and grown up.

To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells. Single cell clones were selected and grown up. The cells were selected with 800ug ml geneticin. The canine gfp lc3 cell line established in this study can be not only used to detect the autophagic activity of dogs but also used to detect other canine animals such as.

Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods. Raw264 7 cells were transfected with pegfp lc3 plasmids. Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods. The raw264 7 cells stably expressing gfp lc3 white were incubated in complete media control and in earle s balanced salts solution starve or simultaneously supplemented with wortmannin starve wort for 1 h.

Rfp acid stable and gfp acid sensitive. Raw264 7 cell line stable expression of gfp lc3 is shown. To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells. The cells were selected with 800ug ml geneticin.

The cells were selected with 800ug ml geneticin. So far many gfp lc3 expressed cell line from different species have been used to measure their autophagy activities including humans other mammals birds and fishes.